Browsing Science (Faculty of) by Title
Now showing items 228-247 of 3011
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Biochemical and Biophysical Investigations of Non-Zinc Dependent Glyoxalase I Enzymes
(University of Waterloo, 2008-09-26)The principal methylglyoxal (MG)-detoxifying system in most living organisms is the two metalloenzyme Glyoxalase system. Glyoxalase I (GlxI) initially converts the non-enzymatically formed MG-GSH hemithioacetal to the ... -
Biochemical and Microbiological Investigations of Inhibitors for β-Lactamases from Human Pathogenic Bacteria
(University of Waterloo, 2019-04-30)The majority of antibiotics prescribed for treatment of bacterial infections are β-lactam antibiotics. Resistance to these has evolved in a few different ways, notably by regulating permeability and by the expression of ... -
Biochemical Characterization and Optimization of RNA Aptamers Bound to Hoechst Dye Derivatives
(University of Waterloo, 2023-08-09)Ribonucleic acid (RNA) aptamers are single-stranded nucleic acids that are typically selected through a process known as Systematic Evolution of Ligands by EXponential Enrichment (SELEX). These oligonucleotides can bind ... -
Biochemical Characterization of a Lanthanide-Dependent DNAzyme with Normal and Phosphorothioate-Modified Substrates
(American Chemical Society, 2015-10-06)A trivalent lanthanide (Ln3+)-dependent RNA-cleaving DNAzyme, Ce13d, was recently isolated via in vitro selection. Ce13d is active in the presence of all Ln3+ ions. Via introduction of a single phosphorothioate (PS) ... -
Biochemical Characterization of Fluorophore-Binding Nucleic Acids
(University of Waterloo, 2022-09-27)Aptamers, single stranded RNA or DNA that bind to target ligands with high affinity and specificity, have a wide range of applications, from therapeutics to biosensing, which makes biochemical characterization of the utmost ... -
Biochemical Investigations of L-Methionine gamma-lyase 1 from Trichomonas vaginalis
(University of Waterloo, 2011-12-16)The enzyme L-methionine γ-lyase (MGL) utilizes a pyridoxal-5’-phosphate-cofactor in order to convert L-methionine to α-ketobutyrate, ammonia and methyl mercaptan. MGL is proposed to be a potential drug target since it is ... -
Biochemistry in Bacterioferritin
(University of Waterloo, 2006)Bacterioferritin, an iron storage protein having a 24-subunit quaternary structure, was used as a model for the study of host-guest interactions and guest encapsulation, making use of its spherical cage-like structure. ... -
BIOCOMPATIBLE SOLID PHASE MICROEXTRACTION
(University of Waterloo, 2006-12-12)Today’s solid phase microextraction (SPME) is a well known technique that combines knowledge from different fields in an attractive, efficient, and economic way. The development of SPME has seen huge growth since its ... -
Biocompatible Solid-Phase Microextraction Nanoelectrospray Ionization: An Unexploited Tool in Bioanalysis
(American Chemical Society, 2015-12-09)In recent years, different geometrical configurations of solid-phase microextraction (SPME) have been directly coupled to mass spectrometry, resulting in benefits such as diminishing matrix effects, improvement of detection ... -
Bioenergetics of mixotrophic metabolisms: A theoretical analysis
(University of Waterloo, 2019-08-29)Many biogeochemical reactions controlling surface water and groundwater quality, as well as greenhouse gas emissions and carbon turnover rates, are catalyzed by microorganisms. Representing the thermodynamic (or bioenergetic) ... -
Biogeochemical cycling of nutrient silicon in a human-impacted large lake nearshore environment (Hamilton Harbour Area of Concern, Lake Ontario, Canada)
(University of Waterloo, 2017-12-14)The biogeochemical cycling of nutrient silicon (Si) through rivers, wetlands, lakes, and artificial reservoirs regulates the magnitude and bioavailability of Si delivered downstream and to the ocean (Frings et al. 2014; ... -
Bioinformatic insights into the diversity and evolution of bacterial toxins
(University of Waterloo, 2020-01-24)Bacterial toxins are a broad category of molecules ranging from small organic compounds and peptides to large multi-domain or multi-meric enzymes. Several important diseases are caused primarily by bacterial toxins including ... -
Bioinformatic Insights into the Impacts of External Stimuli on the Human Airway
(University of Waterloo, 2021-12-21)The airway epithelium represents a critical component of the human lung that helps orchestrate defences against inhaled substances including air pollution, allergens, bacteria, and viruses. To manage these continuous ... -
Biological responses in fish exposed to municipal wastewater treatment plant effluent in situ
(IWA Publishing, 2021-04-16)Effluents from municipal wastewater treatment plants (MWTPs) are complex mixtures of chemicals including endocrine-disrupting compounds (EDCs) and 17α-ethynylestradiol (EE2). The objective of this study was to evaluate ... -
Biological responses in rainbow darter (Etheostoma caeruleum) to changes in municipal wastewater quality in the Grand River
(University of Waterloo, 2017-09-27)This thesis is a synthesis of studies that explore the effects of urbanization on a small-bodied fish species, rainbow darter (Etheostoma caeruleum), in the central Grand River (Ontario, Canada) over time. This watershed ... -
BIOMECHANICAL ALTERATION OF CORNEAL MORPHOLOGY AFTER CORNEAL REFRACTIVE THERAPY
(University of Waterloo, 2006)<strong>Purpose:</strong> Although orthokeratology (non-surgical corneal reshaping, Corneal Refractive Therapy, CRT®) has been used for almost a half century, contemporary CRT's outcomes and mechanisms still require ... -
Biomimetic sensing based on chemically induced assembly of a signaling DNA aptamer on a fluid bilayer membrane
(Royal Society of Chemistry, 2012-04-18)The adenosine aptamer was split into two halves and linked to a fluid liposome surface; addition of adenosine resulted in aptamer assembly, which did not occur if the split aptamer was attached to silica nanoparticles, ... -
Bioorthogonal Modification of the Major Sheath Protein of Bacteriophage M13: Extending the Versatility of Bionanomaterial Scaffolds
(American Chemical Society, 2016-09-04)With a mass of ~1.6 x 107 Daltons and com- posed of approximately 2700 proteins, bacteriophage M13 has been employed as a molecular scaffold in bionanomaterials fabrication. In order to extend the versatility of M13 in ... -
Biophysical Investigations of the Interactions between Calmodulin and Nitric Oxide Synthase Enzymes
(University of Waterloo, 2017-12-15)Nitric Oxide (•NO) is an essential biomolecule that has physiological functions in neurotransmission, vasodilation and immune response. •NO is produced by a family of enzymes known as nitric oxide synthases (NOS). NOS ... -
Biophysical Modelling of Antimicrobial Peptide’s Interactions with Phospholipid and Lipopolysaccharide Membranes
(University of Waterloo, 2019-05-23)Antimicrobial peptides (AMPs) are naturally-occurring peptide antibiotics. The way they work has inspired a vigorous search for optimized peptide antibiotics for fighting resistant bacteria. Cationic AMPs cleverly utilize ...