Now showing items 1-5 of 5
From Metabolite Concentration to Flux – A Systematic Assessment of Error in Cell Culture Metabolomics
(University of Waterloo, 2016-08-05)
The growing availability of genomic, transcriptomic, and metabolomic data has opened the door to the synthesis of multiple levels of information in biological research. As a consequence, there has been a push to analyze ...
Identifying model error in metabolic flux analysis - a generalized least squares approach
(BioMed Central, 2016-09-13)
Background: The estimation of intracellular flux through traditional metabolic flux analysis (MFA) using an overdetermined system of equations is a well established practice in metabolic engineering. Despite the continued ...
Construction and analysis of a genetically tuneable lytic phage display system
The Bacteriophage lambda capsid protein gpD has been used extensively for fusion polypeptides that can be expressed from plasmids in Escherichia coli and remain soluble. In this study, a genetically controlled dual expression ...
A correction method for systematic error in 1H-NMR time-course data validated through stochastic cell culture simulation
(BioMed Central, 2015-09-04)
Background: The growing ubiquity of metabolomic techniques has facilitated high frequency time-course data collection for an increasing number of applications. While the concentration trends of individual metabolites can ...
Treating Cell Culture Media with UV Irradiation against Adventitious Agents: Minimal Impact on CHO Performance
Sterility of cell culture media is an important concern in biotherapeutic processing. In large scale biotherapeutic production, a unit contamination of cell culture media can have costly effects. Ultraviolet (UV) irradiation ...