Browsing Theses by Subject "Bacillus subtilis"
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Application of hydrocarbon and perfluorocarbon oxygen vectors to enhance heterologous production of hyaluronic acid in engineered Bacillus subtilis
(University of Waterloo, 2017-12-19)In microbial cultivations for hyaluronic acid (HA) production, oxygen can be a limiting substrate due to its poor solubility in aqueous medium and the substantial increase in culture viscosity at relatively low HA titers. ... -
A comprehensive CRISPR-Cas9 toolkit for Bacillus subtilis: development for biomanufacturing applications
(University of Waterloo, 2018-04-30)Bacillus subtilis is a model Gram-positive GRAS organism sought after for its robust growth characteristics in inexpensive media, genetic tractability, and ability to secrete products into the extracellular environment. ...