dc.contributor.author | Piccolo, Kyle | |
dc.contributor.author | McNeil, Brooke | |
dc.contributor.author | Crouse, Jeff | |
dc.contributor.author | Lim, Su Ji | |
dc.contributor.author | Hopkins, W. Scott | |
dc.contributor.author | Dieckmann, Thorsten | |
dc.date.accessioned | 2021-10-20 17:48:10 (GMT) | |
dc.date.available | 2021-10-20 17:48:10 (GMT) | |
dc.date.issued | 2020-07-18 | |
dc.identifier.uri | https://doi.org/10.1016/j.bbrc.2020.06.056 | |
dc.identifier.uri | http://hdl.handle.net/10012/17658 | |
dc.description | The final publication is available at Elsevier via http://dx.doi.org/10.1016/j.bbrc.2020.06.056. © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
dc.description.abstract | Binding affinity and selectivity are critical properties of aptamers that must be optimized for any
application. The sulforhodamine B binding RNA aptamer (SRB‐2) is a somewhat promiscuous aptamer
that can bind ligands that vary markedly in shape, size and charge. Here we categorize potential ligands
based on their binding mode and structural characteristics required for high affinity and selectivity.
Several known and potential ligands of SRB‐2 were screened for binding affinity using LSPR, ITC and
NMR spectroscopy. The study shows that rhodamine B has the ideal structural and electrostatic
properties for selective and high‐affinity binding of the SRB‐2 aptamer. | en |
dc.description.sponsorship | NSERC Discovery Grant 303454 | en |
dc.language.iso | en | en |
dc.publisher | Elsevier Inc. | en |
dc.subject | aptamer | en |
dc.subject | ligand | en |
dc.subject | selectivity | en |
dc.subject | binding affinity | en |
dc.subject | RNA | en |
dc.title | Ligand Specificity and Affinity in the Sulforhodamine B Binding RNA Aptamer | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Piccolo KA , McNeil B , Crouse J , Lim SJ , Bickers SC , Hopkins WS , Dieckmann T. (2020). Ligand specificity and affinity in the sulforhodamine B binding RNA aptamer.Biochemical and biophysical research communications. 529(3): 666-671. | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Chemistry | en |
uws.typeOfResource | Text | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |
uws.scholarLevel | Graduate | en |