Now showing items 1-6 of 6
A DNAzyme requiring two different metal ions at two distinct sites
(Oxford University Press, 2015-12-10)
Most previously reported RNA-cleaving DNAzymes require only a single divalent metal ion for catalysis. We recently reported a general trivalent lanthanide-dependent DNAzyme named Ce13d. This work shows that Ce13d requires ...
Liposome/Graphene Oxide Interaction Studied by Isothermal Titration Calorimetry
(American Chemical Society, 2015-03-15)
The interaction between graphene oxide (GO) and lipid bilayers is important for fundamental surface science and many applications. In this work, isothermal titration calorimetry (ITC), cryo-TEM, and fluorescence spectroscopy ...
DNAzyme Hybridization, Cleavage, Degradation and Sensing in Undiluted Human Blood Serum
(American Chemical Society, 2015-04-07)
RNA-cleaving DNAzymes provide a unique platform for developing biosensors. However, a majority of the work has been performed in clean buffer solutions, while the activity of some important DNAzymes in biological sample ...
Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium
(American Chemical Society, 2015-10-20)
Thallium (Tl) is a highly toxic heavy metal situated between mercury and lead in the periodic table. While its neighbors have been thoroughly studied for DNA-based sensing, little is known about thallium detection. In this ...
A Cleavable Molecular Beacon for Hg2+ Detection based on Phosphorothioate RNA Modifications
(American Chemical Society, 2015-06-10)
Mercury is a highly toxic heavy metal, and detection of Hg2+ by biosensors has attracted extensive research interest in the past decade. In particular, a number of DNA-based sensing strategies have been developed. Well-known ...
Biochemical Characterization of a Lanthanide-Dependent DNAzyme with Normal and Phosphorothioate-Modified Substrates
(American Chemical Society, 2015-10-06)
A trivalent lanthanide (Ln3+)-dependent RNA-cleaving DNAzyme, Ce13d, was recently isolated via in vitro selection. Ce13d is active in the presence of all Ln3+ ions. Via introduction of a single phosphorothioate (PS) ...