Now showing items 1-10 of 19
Hydrogel porosity controlling DNA-directed immobilization of gold nanoparticles revealed by DNA melting and scanning helium ion microscopy
(Royal Society of Chemistry, 2012-04-07)
Immobilization of nanomaterials is important for many applications, including sensor development, biomaterials design and catalysis. DNA-directed immobilization has been widely used because of its high specificity and ...
Blue emitting gold nanoclusters templated by poly-cytosine DNA at low pH and poly-adenine DNA at neutral pH
(Royal Society of Chemistry, 2012-07-11)
Blue fluorescent gold nanoclusters were prepared in the presence of poly-cytosine DNAs at low pH and poly-adenine at neutral pH using citrate as the reducing agent; various buffer conditions affecting the synthesis have ...
Amplifying the Macromolecular Crowding Effect Using Nanoparticles
(American Chemical Society, 2012-01-11)
The melting temperature (Tm) of DNA is affected not only by salt but also by the presence of high molecular weight (MW) solutes, such as polyethylene glycol (PEG), acting as a crowding agent. For short DNAs in a solution ...
Biomimetic sensing based on chemically induced assembly of a signaling DNA aptamer on a fluid bilayer membrane
(Royal Society of Chemistry, 2012-04-18)
The adenosine aptamer was split into two halves and linked to a fluid liposome surface; addition of adenosine resulted in aptamer assembly, which did not occur if the split aptamer was attached to silica nanoparticles, ...
Instantaneous and Quantitative Functionalization of Gold Nanoparticles with Thiolated DNA Using a pH-Assisted and Surfactant-Free Route
(American Chemical Society, 2012-05-02)
The attachment of thiolated DNA to gold nanoparticles (AuNPs) has enabled many landmark works in nanobiotechnology. This conjugate chemistry is typically performed using a salt-aging protocol where, in the presence of an ...
Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine
(American Chemical Society, 2012-04-25)
With a low optical background, high loading capacity, and good biocompatibility, hydrogels are ideal materials for immobilization of biopolymers to develop optical biosensors. We recently immobilized mercury and lead binding ...
Adsorption of DNA onto gold nanoparticles and graphene oxide: surface science and applications
(Royal Society of Chemistry, 2012-06-28)
The interaction between DNA and inorganic surfaces has attracted intense research interest, as a detailed understanding of adsorption and desorption is required for DNA microarray optimization, biosensor development, and ...
Ultrahigh Nanoparticle Stability against Salt, pH, and Solvent with Retained Surface Accessibility via Depletion Stabilization
(American Chemical Society, 2012-06-20)
For many applications, it is desirable to stabilize colloids over a wide range of buffer conditions while still retaining surface accessibility for adsorption and reaction. Commonly used charge or steric stabilization ...
Surface Science of DNA Adsorption onto Citrate-Capped Gold Nanoparticles
(American Chemical Society, 2012-02-28)
Single-stranded DNA can be adsorbed by citrate capped gold nanoparticles (AuNPs), resulting in increased AuNP stability, which forms the basis of a number of biochemical and analytical applications, but the fundamental ...
DNA-Length-Dependent Fluorescence Signaling on Graphene Oxide Surface
Fluorescence energy transfer to graphene oxide is studied using covalently linked DNA probes ranging from 4 to 70 base pairs. The characteristic distance and mechanism of energy transfer are reported.