Now showing items 1-10 of 39
Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing
(American Chemical Society, 2016-10-18)
Fluorescently labeled DNA adsorbed on graphene oxide (GO) is a well-established sensing platform for detecting a diverse range of analytes. GO is a loosely defined material and its oxygen content may vary depending on the ...
G-Quadruplex DNA for Fluorescent and Colorimetric Detection of Thallium(I)
(American Chemical Society, 2016-02-26)
Thallium is a highly toxic heavy metal, but its 495 nm FRET sensing is underexplored compared to its neighboring elements Blue in the periodic table: lead and mercury. Thallium has two " oxidation states. A DNAzyme-based ...
Liposome/Graphene Oxide Interaction Studied by Isothermal Titration Calorimetry
(American Chemical Society, 2015-03-15)
The interaction between graphene oxide (GO) and lipid bilayers is important for fundamental surface science and many applications. In this work, isothermal titration calorimetry (ITC), cryo-TEM, and fluorescence spectroscopy ...
Covalent linking DNA to graphene oxide and its comparison with physisorbed probes for Hg2+ detection
Graphene oxide (GO) has attracted extensive research interest as a platform for DNA adsorption and biosensor development. While most researchers use simple physisorption of fluorescently labeled DNA, covalent sensors are ...
A DNAzyme requiring two different metal ions at two distinct sites
(Oxford University Press, 2015-12-10)
Most previously reported RNA-cleaving DNAzymes require only a single divalent metal ion for catalysis. We recently reported a general trivalent lanthanide-dependent DNAzyme named Ce13d. This work shows that Ce13d requires ...
An Ultrasensitive Light-up Cu2+ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate
(American Chemical Society, 2017-01-15)
Cu2+ is a very important metal ion in biology, environmental science, and industry. Developing biosensors for Cu2+ is a key topic in analytical chemistry. DNAzyme-based sensors are highly attractive:, for their excellent ...
DNA-Length-Dependent Fluorescence Signaling on Graphene Oxide Surface
Fluorescence energy transfer to graphene oxide is studied using covalently linked DNA probes ranging from 4 to 70 base pairs. The characteristic distance and mechanism of energy transfer are reported.
Molecular Beacon Lighting up on Graphene Oxide
(American Chemical Society, 2012-05-01)
A molecular beacon (MB) is comprised of a fluorophore and a quencher linked by a DNA hairpin. MBs have been widely used for homogeneous DNA detection. In addition to molecular quenchers, many nanomaterials such as graphene ...
In Vitro Selection of a DNAzyme Cooperatively Binding Two Lanthanide Ions for RNA Cleavage
(American Chemical Society, 2016-05-03)
Trivalent lanthanide ions (Ln(3+)) were recently employed to select RNA-cleaving DNAzymes, and three new DNAzymes have been reported so far. In this work, dysprosium (Dy3+) was used with a library containing 50 random ...
DNA Adsorption by ZnO Nanoparticles near Its Solubility Limit: Implications for DNA Fluorescence Quenching and DNAzyme Activity Assays
(American Chemical Society, 2016-06-07)
Zinc oxide (ZnO) is a highly important material, and Zn2+ is a key metal ion in biology. ZnO and Zn2+ interconvert via dissolution and hydrolysis/condensation. In this work, we explore their interactions with DNA, which ...