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Searching for a DNAzyme version of the leadzyme
The leadzyme refers to a small ribozyme that cleaves a RNA substrate in the presence of Pb2+. In an optimized form, the enzyme strand contains only two unpaired nucleotides. Most RNA-cleaving DNAzymes are much longer. Two ...
Biochemical Characterization of a Lanthanide-Dependent DNAzyme with Normal and Phosphorothioate-Modified Substrates
(American Chemical Society, 2015-10-06)
A trivalent lanthanide (Ln3+)-dependent RNA-cleaving DNAzyme, Ce13d, was recently isolated via in vitro selection. Ce13d is active in the presence of all Ln3+ ions. Via introduction of a single phosphorothioate (PS) ...
Tandem DNAzymes for mRNA cleavage: choice of enzyme, metal ions and the antisense effect
The concept of DNAzyme-based gene silencing via mRNA cleavage was proposed over twenty years ago. A number of studies regarding intracellular gene silencing have been reported as well. However, questions have been raised ...
Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium
(American Chemical Society, 2015-10-20)
Thallium (Tl) is a highly toxic heavy metal situated between mercury and lead in the periodic table. While its neighbors have been thoroughly studied for DNA-based sensing, little is known about thallium detection. In this ...