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A DNAzyme requiring two different metal ions at two distinct sites
(Oxford University Press, 2015-12-10)
Most previously reported RNA-cleaving DNAzymes require only a single divalent metal ion for catalysis. We recently reported a general trivalent lanthanide-dependent DNAzyme named Ce13d. This work shows that Ce13d requires ...
Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing
(American Chemical Society, 2016-10-18)
Fluorescently labeled DNA adsorbed on graphene oxide (GO) is a well-established sensing platform for detecting a diverse range of analytes. GO is a loosely defined material and its oxygen content may vary depending on the ...
G-Quadruplex DNA for Fluorescent and Colorimetric Detection of Thallium(I)
(American Chemical Society, 2016-02-26)
Thallium is a highly toxic heavy metal, but its 495 nm FRET sensing is underexplored compared to its neighboring elements Blue in the periodic table: lead and mercury. Thallium has two " oxidation states. A DNAzyme-based ...
Liposome/Graphene Oxide Interaction Studied by Isothermal Titration Calorimetry
(American Chemical Society, 2015-03-15)
The interaction between graphene oxide (GO) and lipid bilayers is important for fundamental surface science and many applications. In this work, isothermal titration calorimetry (ITC), cryo-TEM, and fluorescence spectroscopy ...
Covalent linking DNA to graphene oxide and its comparison with physisorbed probes for Hg2+ detection
(Elsevier, 2016-05-15)
Graphene oxide (GO) has attracted extensive research interest as a platform for DNA adsorption and biosensor development. While most researchers use simple physisorption of fluorescently labeled DNA, covalent sensors are ...
DNA Adsorption by ZnO Nanoparticles near Its Solubility Limit: Implications for DNA Fluorescence Quenching and DNAzyme Activity Assays
(American Chemical Society, 2016-06-07)
Zinc oxide (ZnO) is a highly important material, and Zn2+ is a key metal ion in biology. ZnO and Zn2+ interconvert via dissolution and hydrolysis/condensation. In this work, we explore their interactions with DNA, which ...
Distinction of Individual Lanthanide Ions with a DNAzyme Beacon Array
(American Chemical Society, 2016-06-24)
Developing chemical probes to distinguish each lanthanide ion is a long-standing challenge. Aside from its analytical applications, solving this problem will also enhance our knowledge in metal ligand design. Using in vitro ...
Hg2+ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA
(Royal Society of Chemistry, 2016-06-21)
Mercury is a highly toxic heavy metal and many DNA-based biosensors have been recently developed for Hg2+ detection in water. Among them, thymine-rich DNA is the most commonly used for designing Hg2+ sensors. However, the ...
In Vitro Selection of New DNAzymes as Metal Biosensors
(University of Waterloo, 2015-06-26)
In vitro selection of metal-specific DNAzymes has been shown to be a powerful method to obtain biosensors for metal ion detection. With 14 independent in vitro selection experiments using each of the trivalent lanthanide ...
Citrate inhibition of cisplatin reaction with DNA studied using fluorescently labeled oligonucleotides: implication for selectivity towards guanine
(Royal Society of Chemistry, 2013-10-21)
The reaction between cisplatin and DNA is conveniently studied using fluorescently labeled oligonucleotides and gel electrophoresis; as an example of application, the inhibition of this reaction by citrate is demonstrated, ...