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DNA Adsorption, Desorption, and Fluorescence Quenching by Graphene Oxide and Related Analytical Application
(University of Waterloo, 2011-09-20)
Graphene is a single layer of graphite with many unique mechanical, electrical, and optical properties. In addition, graphene is also known to adsorb wide range of biomolecules including single-stranded DNA. On the other ...
DNAzyme Hybridization, Cleavage, Degradation and Sensing in Undiluted Human Blood Serum
(American Chemical Society, 2015-04-07)
RNA-cleaving DNAzymes provide a unique platform for developing biosensors. However, a majority of the work has been performed in clean buffer solutions, while the activity of some important DNAzymes in biological sample ...
DNA Adsorption by ZnO Nanoparticles near Its Solubility Limit: Implications for DNA Fluorescence Quenching and DNAzyme Activity Assays
(American Chemical Society, 2016-06-07)
Zinc oxide (ZnO) is a highly important material, and Zn2+ is a key metal ion in biology. ZnO and Zn2+ interconvert via dissolution and hydrolysis/condensation. In this work, we explore their interactions with DNA, which ...
Distinction of Individual Lanthanide Ions with a DNAzyme Beacon Array
(American Chemical Society, 2016-06-24)
Developing chemical probes to distinguish each lanthanide ion is a long-standing challenge. Aside from its analytical applications, solving this problem will also enhance our knowledge in metal ligand design. Using in vitro ...
In Vitro Selection of a DNAzyme Cooperatively Binding Two Lanthanide Ions for RNA Cleavage
(American Chemical Society, 2016-05-03)
Trivalent lanthanide ions (Ln(3+)) were recently employed to select RNA-cleaving DNAzymes, and three new DNAzymes have been reported so far. In this work, dysprosium (Dy3+) was used with a library containing 50 random ...
Hg2+ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA
(Royal Society of Chemistry, 2016-06-21)
Mercury is a highly toxic heavy metal and many DNA-based biosensors have been recently developed for Hg2+ detection in water. Among them, thymine-rich DNA is the most commonly used for designing Hg2+ sensors. However, the ...
Nucleotide coordination with 14 lanthanides studied by isothermal titration calorimetry
(Elsevier, 2017-06-09)
With their hydrolytic, optical and magnetic properties, lanthanide ions (Ln3+) are versatile probes for nucleic acids. In addition, nucleotide-coordinated Ln3+ ions form useful nanoparticles. However, the thermodynamic ...
A DNAzyme requiring two different metal ions at two distinct sites
(Oxford University Press, 2015-12-10)
Most previously reported RNA-cleaving DNAzymes require only a single divalent metal ion for catalysis. We recently reported a general trivalent lanthanide-dependent DNAzyme named Ce13d. This work shows that Ce13d requires ...
Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing
(American Chemical Society, 2016-10-18)
Fluorescently labeled DNA adsorbed on graphene oxide (GO) is a well-established sensing platform for detecting a diverse range of analytes. GO is a loosely defined material and its oxygen content may vary depending on the ...
An Ultrasensitive Light-up Cu2+ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate
(American Chemical Society, 2017-01-15)
Cu2+ is a very important metal ion in biology, environmental science, and industry. Developing biosensors for Cu2+ is a key topic in analytical chemistry. DNAzyme-based sensors are highly attractive:, for their excellent ...