Now showing items 1-10 of 40
DNA Adsorption, Desorption, and Fluorescence Quenching by Graphene Oxide and Related Analytical Application
(University of Waterloo, 2011-09-20)
Graphene is a single layer of graphite with many unique mechanical, electrical, and optical properties. In addition, graphene is also known to adsorb wide range of biomolecules including single-stranded DNA. On the other ...
In Vitro Selection of New DNAzymes as Metal Biosensors
(University of Waterloo, 2015-06-26)
In vitro selection of metal-specific DNAzymes has been shown to be a powerful method to obtain biosensors for metal ion detection. With 14 independent in vitro selection experiments using each of the trivalent lanthanide ...
Functional nucleic acids for detecting bacteria
(De Gruyter, 2013-01-31)
Bacterial infection represents one of the leading causes of disease and death, and as such, bacterial detection is an important step in managing infectious diseases. The current protocol requires growing cell cultures, ...
Extraction of DNA staining dyes from DNA using hydrophobic ionic liquids
(Royal Society of Chemistry, 2013-05-18)
The partition of oligonucleotides and DNA staining dyes into a few hydrophobic ionic liquids has been studied, where the oligonucleotides remain in the aqueous phase and all the DNA staining dyes are extracted in the ionic ...
Oxidation Level-Dependent Zwitterionic Liposome Adsorption and Rupture by Graphene-based Materials and Light-Induced Content Release
Liposomes may be stably adsorbed or ruptured on graphene-based materials, depending on the oxidation state of graphene. IR-induced liposome leakage is achieved, since graphene oxide does not induce liposome leakage spontaneously.
G-Quadruplex DNA for Fluorescent and Colorimetric Detection of Thallium(I)
(American Chemical Society, 2016-02-26)
Thallium is a highly toxic heavy metal, but its 495 nm FRET sensing is underexplored compared to its neighboring elements Blue in the periodic table: lead and mercury. Thallium has two " oxidation states. A DNAzyme-based ...
Liposome/Graphene Oxide Interaction Studied by Isothermal Titration Calorimetry
(American Chemical Society, 2015-03-15)
The interaction between graphene oxide (GO) and lipid bilayers is important for fundamental surface science and many applications. In this work, isothermal titration calorimetry (ITC), cryo-TEM, and fluorescence spectroscopy ...
An Ultrasensitive Light-up Cu2+ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate
(American Chemical Society, 2017-01-15)
Cu2+ is a very important metal ion in biology, environmental science, and industry. Developing biosensors for Cu2+ is a key topic in analytical chemistry. DNAzyme-based sensors are highly attractive:, for their excellent ...
Covalent linking DNA to graphene oxide and its comparison with physisorbed probes for Hg2+ detection
Graphene oxide (GO) has attracted extensive research interest as a platform for DNA adsorption and biosensor development. While most researchers use simple physisorption of fluorescently labeled DNA, covalent sensors are ...
Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing
(American Chemical Society, 2016-10-18)
Fluorescently labeled DNA adsorbed on graphene oxide (GO) is a well-established sensing platform for detecting a diverse range of analytes. GO is a loosely defined material and its oxygen content may vary depending on the ...