Browsing University of Waterloo by Author "Palmer, Michael"
Now showing items 1-14 of 14
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The action mechanism of daptomycin
Taylor, Scott D.; Palmer, Michael (Elsevier, 2016-12-15)Daptomycin is a lipopeptide antibiotic produced by the soil bacterium Streptomyces roseosporus that is clinically used to treat severe infections with Gram-positive bacteria. In this review, we discuss the mode of action ... -
An Acyl-Linked Dimer of Daptomycin Is Strongly Inhibited by the Bacterial Cell Wall
Taylor, Robert; Scott, Bradley; Taylor, Scott D.; Palmer, Michael (American Chemical Society, 2017-03-28)The lipopeptide antibiotic daptomycin is active against Gram-positive pathogens. It permeabilizes bacterial cell membranes, which involves the formation of membrane-associated oligomers. We here studied a dimer of daptomycin ... -
Cardiolipin Prevents Membrane Translocation and Permeabilization by Daptomycin
Zhang, TianHua; Muraih, Jawad K.; Tishbi, Nasim; Herskowitz, Jennifer; Victor, Rachel L.; Silverman, Jared; Uwumarenogie, Stephanie; Taylor, Scott D.; Palmer, Michael; Mintzer, Evan (American Society for Biochemistry and Molecular Biology, 2014-04-25)Daptomycin is an acidic lipopeptide antibiotic that, in the presence of calcium, forms oligomeric pores on membranes containing phosphatidylglycerol. It is clinically used against various Gram-positive bacteria such as ... -
Characterization of daptomycin oligomerization with perylene excimer fluorescence: Stoichiometric binding of phosphatidylglycerol triggers oligomer formation
Muraih, Jawad K.; Harris, Jesse; Taylor, Scott D.; Palmer, Michael (Elsevier, 2012-03)Daptomycin is a lipopeptide antibiotic that binds to and depolarizes bacterial cell membranes. Its antibacterial activity requires calcium and correlates with the content of phosphatidylglycerol in the target membrane. ... -
Combination treatment of epilepsy with ketogenic diet and concurrent pharmacological inhibition of cytochrome P450 2E1
Palmer, Michael (Elsevier, 2013-04)While most epileptic patients respond to treatment with existing antiepileptic drugs, there remains a considerable number of patients in whom these drugs do not suffice. Such patients, particularly children, are often ... -
Daptomycin forms cation- and size-selective pores in model membranes
Zhang, TianHua; Muraih, Jawad K.; MacCormick, Ben; Silverman, Jared; Palmer, Michael (Elsevier, 2014-10)Daptomycin is a lipopeptide antibiotic that is used clinically to treat severe infections caused by Gram-positive bacteria. Its bactericidal action involves the calcium-dependent binding to membranes containing ... -
Estimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRET
Muraih, Jawad K.; Palmer, Michael (Elsevier, 2012-07)Daptomycin is a lipopeptide antibiotic that kills Gram-positive bacteria by depolarizing their cell membranes. This antibacterial action of daptomycin is correlated with the formation of membrane-associated oligomers. We ... -
Mechanistic studies on the effect of membrane lipid acyl chain composition on daptomycin pore formation
Beriashvili, David; Taylor, Robert; Kralt, Braden; Abu Mazen, Nooran; Taylor, Scott D.; Palmer, Michael (Elsevier, 2018-11-01)Daptomycin is a lipopeptide antibiotic that binds and permeabilizes the cell membranes of Gram-positive bacteria. Membrane permeabilization requires both calcium and phosphatidylglycerol (PG) in the target membrane, and ... -
Membrane binding and oligomer formation by the calcium-dependent lipopeptide antibiotic A54145: a quantitative study with pyrene excimer fluorescence
Zhang, TianHua; Taylor, Scott D.; Palmer, Michael; Duhamel, Jean (Elsevier, 2016-09-20)A54145 is a lipopeptide antibiotic related to daptomycin that permeabilizes bacterial cell membranes. Its action requires both calcium and phosphatidylglycerol in the target membrane, and it is accompanied by the formation ... -
mol2chemfig, a tool for rendering chemical structures from molfile or SMILES format to LATE X code
Brefo-Mensah, Eric K.; Palmer, Michael (Springer, 2012-10-02)Displaying chemical structures in LATE X documents currently requires either hand-coding of the structures using one of several LATE X packages, or the inclusion of finished graphics files produced with an external drawing ... -
Mutual inhibition through hybrid oligomer formation of daptomycin and the semisynthetic lipopeptide antibiotic CB-182,462
Zhang, TianHua; Mintzer, Evan; Tishbi, Nasim; Desert, Celine; Silverman, Jared; Taylor, Scott D.; Palmer, Michael (Elsevier, 2013-02)Daptomycin is a clinically important lipopeptide antibiotic that kills Gram-positive bacteria through membrane depolarization. Its activity requires calcium and the presence of phosphatidylglycerol in the target membrane. ... -
Oligomerization and hemolytic properties of the C-terminal domain of pyolysin, a cholesterol-dependent cytolysin
Pokrajac, Lisa A.; Harris, J. Robin; Sarraf, Naghmeh S.; Palmer, Michael (NRC Research Press, 2013-04)Pyolysin (PLO) belongs to the homologous family of the cholesterol-dependent cytolysins (CDCs), which bind to cell membranes containing cholesterol to form oligomeric pores of large size. The CDC monomer structure consists ... -
Partial oligomerization of pyolysin induced by a disulfide-tethered mutant
Pokrajac, Lisa A.; Baik, Clara; Harris, J. Robin; Sarraf, Naghmeh S.; Palmer, Michael (NRC Research Press, 2012-12)The bacterial toxin pyolysin (PLO) belongs to the family of cholesterol-dependent cytolysins (CDCs), which form large, ring-shaped oligomeric pores in cholesterol-containing membranes. Monomeric CDC molecules have a structure ... -
Two successive calcium-dependent transitions mediate membrane binding and oligomerization of daptomycin and the related antibiotic A54145
Taylor, Robert; Butt, Khalida; Scott, Bradley; Zhang, TianHua; Muraih, Jawad K.; Mintzer, Evan; Taylor, Scott D.; Palmer, Michael (Elsevier, 2016-09)Daptomycin and A54145 are homologous lipopeptide antibiotics that permeabilize the cell membranes of Gram-positive bacteria. Membrane permeabilization depends on the presence of both phosphatidylglycerol (PG) and calcium, ...